Publications from the vSEED project
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- Dekkers, B., Willems, L., Bassel, G.W., van Bolderen-Veldkamp, R.P., Ligterink, W., Hilhorst, H.W.M., Bentsink L. Identification Of Reference Genes For RT-qPCR Expression Analysis In Arabidopsis And Tomato Seeds (2011) Plant Cell Physiol.. pcr113 first published online August 18, 2011 doi:10.1093/pcp/pcr113. A genome-wide network model generated using rule-based machine learning. Functional associations between genes are established by the collective ability to come together and form "rules" that accurately predict developmnetal outcome in seeds. This paper looks at how an elastic membrane deforms when a rigid indenter is pushed into it. The membrane is allowed to have an arbitrary shape and elastic properties, while the indenter may also take any convex shape.In this manuscript we generated and analysed a genome-wide network model using publicly available data that describes global transcriptional interactions in Arabidopsis seeds that we term “SeedNet”, and show that SeedNet represents a step-change in our understanding of the regulation of germination. Here we have used the freely available PageMan/MapMan package (http://MapMan.gabipd.org) to visualize transcriptome and metabolome changes in Arabidopsis thaliana seeds during dormancy and germination. Using this package we developed two seed-specific MapMan pathways, which efficiently capture the most important molecular processes in seeds. Darwin review about the early phase of seed germination Developmental processes like seed germination are characterised by massive transcriptome changes. This study compares seed transcriptome datasets of different Brassicaceae to identify stable expressed reference genes for cross-species qRT-PCR normalisation. A workflow is presented for improving RNA quality, qRT-PCR performance, and normalisation when analysing expression changes across species. |